Figure 3.The mRNA expression of VEGF, Tie1 and aFGF in islets exposed to hyperoxia, normoxia or normoxia. Isolated islets, 100 per group, were exposed to hyperoxia (100% O2), normoxia (21% O2) or hypoxia (1% O2) for six hours. mRNA expression was analyzed by RT-PCR and agarose gel electrophoresis. The relative mRNA expression was expressed as percentage of control, where mRNA expression at 100% oxygen was used as control. The results are expressed as mean±SEM..