Figure 1: Workflow of the quantitative mass spectrometry-based TMT MS3 experiments. Following cell lysis and protein extraction, proteins were digested with LysC and then trypsin. Each sample was labeled with a specific TMT isobaric tag (control cells were labeled with 126-128 colored in blue and PanC1 with 129-131 colored in red) and then combined. The pooled sample was fractionated by basic pH reversed-phase (BpRP) liquid chromatography and subjected to LC-MS3 analysis. This procedure was performed in parallel for both HPNE and PanC1 cell lines.