Figure 2: In response to the growth factor KGF, the intra islet precursor from the epithelial cell sheet starts differentiating into islet like clusters which primarily form an outgrowth-like structure within the cell sheet as shown by the arrow (Figure 2a). The islet bud turns into a well-defined islet in another 4 days under the influence of KGF. Specificity of the neogenerated islets of varying size were examined by islet specific stain DTZ; neogenerated islets appeared crimson red in colour (islets shown by arrow marks) after DTZ staining (Figure 2b).