Figure 3. The minimum cytotoxic dose of NDV-LS required for normal human cells (a.) or pancreatic cancer cells (b.) cultured with (+) or without (-) acetylated trypsin (AT). The bars represent means±SEM. With acetylated trypsin, the minimum cytotoxic dose of NDV-LS in all pancreatic cancer lines studied was decreased by half (P< 0.001, Wilcoxon matched-pairs test). Similarly, the cytotoxic dose with or without acetylated trypsin in normal control lines was decreased by 64% (P< 0.001, Wilcoxon matched-pairs test). Without acetylated trypsin, HPDE or HuFb or HPDE or HEKn cells (n=10, 15, 11, 10, respectively) were significantly less sensitive to NDV-LS cytotoxicity as compared to Panc 10.05, PL45, PANC-1, SU.86.86, or BxPC3 cells (P< 0.01, Kruskal-Wallis with Dunn’s multiple comparison tests; n=16, 14, 24, 15, 12, respectively).