Figure 5: Expression of neo mRNA in spleen cells of BB rats treated 6 months previously with either untransduced cells or with MSCVneo.IL-4 transduced spleen cells. Total RNA was extracted from the spleens of treated rats and expression of the neo marker gene was determined by RT-PCR β-Actin was used as a positive control. PCR products were visualized on 2% agarose gels with ethidium bromide.