Trichomonas vaginalis is in some cases found in Pap spreads where it is accounted for, but since accentuation is set on dangerous cells in Pap spreads, very little is done to look for this parasite in spreads. In this analysis, cervical and vaginal examples were inspected infinitesimally in wet arrangements and at the same time by the traditional Papanicolaou strategy for the nearness of Trichomonas vaginalis. The examples were likewise refined for the nearness of Trichomonas vaginalis and PCR done to affirm the nearness of the life forms. At the point when contrasted and positive aftereffects of culture and PCR, wet arrangements had the most noteworthy affectability of 81.58% followed by 65.77% of determination dependent on both perinuclear corona and T. vaginalis. Possible finding dependent on perinuclear radiance alone was 52.63% while conclusion dependent on ID of life forms in Pap smear was 42.11%. Along these lines, the adequacy of conclusion of T. vaginalis in Pap spreads is about 65.77% and it ought not be utilized to reject trichomoniasis.