The aim of this study was to determine the effects of coenzyme Q10 Supplementation on Footsal competitioninduced changes in serum neutrophil in male players. Therefore, Twenty four male footsal player of league(age23/5±1year, height174/75±5.2cm,body mass 71/3±3.5 kg and BMI 22/72±1) were selected and randomly assigned in four groups:( coenzyme Q10, competition + coenzyme Q10,competition + placebo and (placebo) . Based on research design, players took coenzyme supplement (100 mg coenzyme Q10) or placebo three times daily .The players accomplished three successive footsal competitions in time period of 4-6 p.m. The blood samples were collected for analysis of serum neutrophil at the rest status before, immediately and 24 hours after the final session of competitions. data were analyzed by repeated measure ANOVA,LSD post hoc test at P<0/05. Results showed that effect of supplementary consumption of coenzyme Q10 on neutrophil levels during three consecutive matches in competition group with supplement was significantly (p=0/0001) different from other groups. Post hoc test revealed that, this difference was significant in the second and third stages. Results of within group comparison in ANOVA analysis showed that the level of neutrophils in competition group with coenzyme Q10 was significant (p=0.010) in three stages of measurement. The post hoc test indicated that level of significance were increased (p=0.001) in second stage and decreased in third (p=0.027).Based on the results consumption of coenzyme Q10 supplement at the time of successive footsal competitions can reduce serum neutrophil of male footsal players, so can have a beneficial effect on players’ immune system. It is suggested that, Footsal players use coenzyme Q10 supplement during consecutive matches.