The paper investigates and reports siderophores production from bacteria isolated from aquatic environment. In the experimental findings, out of 125 bacterial isolates only twelve morphologically different bacterial cultures were isolated and identified as Escherichia coli(6 isolates, strains CH1-CH6), Pseudomonas aeruginosa(6 isolates, strains CH7-CH12) all of which showed siderophores production by Chrome Azurol Sulphonate(CAS) assay. E.coli Strain CH6showed maximum siderophores production (46%) and Pseudomonas aeruginosa strain CH8 showed the lowest production of siderophore (10%). Siderophores produced by the E.coli strain (strains CH1-CH6) were hydroxymate type and those produced by P. aeruginosa(strains CH7-CH12) were catecholate type. E.coli strain CH6 was chosen for further examination as it showed maximum siderophore production on succinate medium (75%) with10mM Fe at optimum pH 7.0. The prime interest of this experimentation is to further explore and reexamine the biological application in the current Indian context of Agricultural development.