In the present study fresh and market roots of drug Desmodium gangeticum DC. were analyzed for study in changes of chemical constituents under storage. Root samples were stored under different 30, 50, 75, 96 and 100 % relative humidity and different incubation days 15, 30, 45 and 60 days. Quantitative estimation of proteins, phenol and alkaloids in fresh and market roots was done. The results indicated that biodeterioration of selected chemical constituents were observed under high relative humidities 75, 96 and 100% RH and with increased in times of incubation 45 and 60 days. More deterioration of chemical constituents recorded in case of market samples as compared to fresh samples. Analysis of variance also showed that the effect of relative humidity and incubation days on biodeterioration of chemical constituents amount were significant.