The present study was carried out to evaluate the extracts of T. cordifolia was done by using standard procedures. The antibacterial activity of the aqueous extract of leaves of T. cordifolia on four pathogenic bacteria namely Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Enterococcus faecalis for Urinary tract infection (UTI). The leaves of T. cordifolia were powdered and subjected to aqueous extraction. Preliminary phytochemical screening was done by using standard procedures. The antibacterial activity of the aqueous extract of leaves of T. cordifolia was studied using disc diffusion method against Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Enterococcus faecalis these were isolated from 70 school going healthy children. The aqueous extracts of T. cordifolia exhibited effective antimicrobial activity against all four pathogenic bacteria. More specifically, higher percent growth inhibition was obtained in presence of aqueous extracts in comparison to solvent extracts, which was much higher than synthetic antibiotics while the leaf aqueous extract of T. cordifolia exhibited maximum zone of inhibition against Escherichia coli, (28 mm) Pseudomonas aeruginosa (26 mm), Klebsiella pneumoniae (23 mm) and Enterococcus faecalis (22 mm). The present results suggest that the aqueous extracts of T. cordifolia have significant antibacterial activity against Urinary tract infection (UTI) related opportunistic bacterial pathogens.