Berberis tinctoria Lesch. is an evergreen species endemic to the high hills of Nilgiris and has high medicinal value. The present study was carried out to understand the phytochemical constituents of the bark part. The results of the GC-MS analysis carried out in three different extracts viz Acetone, Methanol and Ethyl acetate which exhibited the presence of 22 major phytocomponents. The study also highlighted the presence of some known biologically active phytocomponents like berberine, phytol and stigmasterol etc. Hence present study will further validate the medicinal potentiality of this plant.