ABIDA RAFIQUE
The present study is concerned with the production of antitumor antibiotics from Streptomyces griseocarneus NRRL B1068 by submerged fermentation technique. The antibiotic activity was tested against A.niger, E.coli and B.subtilis by agar well diffusion method. Different culture media were screened and M1 medium consisting of (g/L), potassium dihydrogen phosphate, 3.24; dipotassium hydrogen phosphate, 5.65; hydrated magnesium sulphate, 1.0; and 1 ml stock solution of salts (ferrous sulphate, 0.1; manganese chloride, 0.1; and zinc sulphate, 0.1) supplemented with 7.5% glucose and 2.0% lysine was found to be best medium for fermentation. Optimum temperature, pH and incubation period for the production of antitumor antibiotic were found to be 30 ºC, 7.2 and 7 days, respectively.7 days old inoculum at a concentration of 8% (v/v) were found to be the best for antitumor antibiotic production by Streptomyces griseocarneus NRRL B1068.
