Fifty isolates capable of producing phytase were isolated from various soil samples. Out of fifty isolates five isolates showed maximum phytase activity. P6 isolate was selected for further study P6 isolate was biochemically characterized as Pseudomonas spp. The culture conditions were optimized for maximum enzyme production. The best carbon and nitrogen sources for maximum phytase production were 1% glucose and 0.5% yeast extract respectively. The enzyme was stable between the pH 4 to 10 but the optimal pH was found to be 6. The enzyme was also stable between temperature ranges 30° C to 50°C but best temperature for enzyme activity was found to be 37° C. Maximum phytase activity was 98.76 U/ml after 24 hrs of incubation under optimal conditions. The specific activity of the crude enzyme was 31.86Umg-1 protein and this was increased to 70.77Umg-1 protein by (NH4)2SO4 precipitation.