Influence of light quality (intensity, spectral distribution and transmission wavelength maxima) on regeneration efficiency and microshoot quality is described. We present a prospective potential to modify regeneration efficiency and microshoot quality by varying the quality of incident light. Light quality was evaluated by means of three tinted (blue, yellow & red) and one transparent culture container (control) all receiving light in the range of 400-700 nm. Primordia, number of microshoot, shoot and internodal length were significantly higher in yellow containers. Whereas the number of nodes and leaf length was significantly higher in blue containers but with lowest green coloration. On the other hand, significantly higher green coloration of shoots was observed only in control containers. Induction in light/dark and regeneration on two BAP levels had no consequence, indicative of light quality as the inimitable attribute influencing regeneration efficiency and microshoot quality in chrysanthemum Dendranthema grandiflora.