Mohammad Narimani-Rad, Mortaza Kiyani Nahand, Habib Aghdam Shahryar, Naser Maheri-Sis, Ramin Salamatdoust nobar, Alireza Lotfi
The aim of present study was to investigation on influence of supplemented sunflower oil (SFO) in vitro gas production of mixed ration (concentrated feed + forage) for ruminants. The SFO in the levels of 0, 2.5 or 5% of ration was added to experimental mixed ration via spraying to milled ration. Three native bulls were fistulated and fed with experimental ration twice daily for 15 days and ruminal fluid was collected. Gas production was measured as the volume of gas in the calibrated syringes and was recorded before incubation 2, 4, 6, 8, 12, 24, 48, 72 and 96 hours after incubation. Total gas values were corrected for blank incubation which contained only rumen fluid. For determination of metabolizable energy (ME), net energy for lactation (NEL) and digestibility of organic matter (DOM) in in vitro conditions, an equation was applied for gas production volume from a milligram of sample and turned it for 200 mg sample to 24h. With inclusion of 5% SFO, significant decreases (p<0.05) in gas production volume and both of soluble (a) or insoluble (b) fractions were observed. It was concluded dietary inclusion of 5% sunflower oil may cause considerable decreases in <i>in vitro</i> gas production parameters and energy indices of mixed ration (40%: forage and 60% concentrated feed) for ruminant.
