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Immunocytochemical detection of the Krebs cycle enzyme in infertile male

The aim of the study was to evaluation of succinic dehydrogenase in human semen samples. A total of 45 asthenozoospermia infertile patients and 25 fertile males participated in this study. Immunocytochemical detection was used to localize succinic dehydrogenase (SDH) in the head & mid-piece of spermatozoa by fluorescent microscope. The study revealed dispersed SDH activity in the semen of asthenozoospermic subjects in comparison to control semen samples. While the stain intensity varied from cell to cell in controls as well as in patients, it was predominantly localized with in mid-piece of spermatozoa in healthy individuals. In contrast, in semen of infertile male, the diffused and dispersed SDH immuno-reactivity was observed in head as well as in mid-piece region of spermatozoa. A comparison of staining intensity revealed negative, dispersed-mild, moderate and intense staining in 8.9%, 17.8%, 33.3% and 40% of

Author(s): Rashmi Rana, Nayan K Mohanty1 , Arun K Jain2 Dept. of Research, Sir Ganga Ram Hospital, New Delhi-110060.India, Dept. of Urology, Safdarjung Hospital, New Delhi-110029. India National Institute of Pathology (ICMR) Safdarjang Hospital, New Delhi-110029.India

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