Saponin rich fraction of Chlorophytum borivilianum was investigated for transdermal enhancer activity by using human cadaver skin in vitro with pramipexol as the model drug. Moreover, FT-IR studies were conducted to understand to possible enhancement mechanism. Results shows significantly increased flux of the drug compared to control (p<0.05). Similarly permeability coefficient (Kp), cumulative amount release (Q24) and enhancement ratio (ER) shown significant increase over control sample. FT-IR studies reveal that Chlorophytum borivilianum reduces peak area by 89.00 % for symmetric and asymmetric stretching peaks. In addition significantly reduces percentage of secondary structures of keratin at amide I band. These results indicate that C. borivilianum enhances transdermal permeation of pramipexol by biphasic mechanism involving partial extraction of stratum corneum (SC) lipid and interaction with SC keratin.