The main purpose of this research is analyzing the effect of creatine supplementation combined with aerobic training on anaerobic threshold in young men. The following study is a quasi-experimental study. The population of this study is formed by young healthy men of Shahrood city who do recreational activities. The primarily samples were volunteer selected. A group of 32 young healthy men (between the ages of 18 to 30) agreed to participate in this research. The study was done in 4 weeks and the data needed for a four-week-course was collected before and after the test. The statistical analysis was performed by “SPSS ver.16”. To analyze the data, ANOVA variance test was used. The results showed that using creatine over the course of four weeks, along with aerobic exercise, has affected anaerobic threshold (p=0.02The researcher concluded that creatin supplementation can be effected on anaerobic threshold, But more studies for longer periods (over 4 weeks) are necessary.