Dhamia K. Suker and Reyam Moaid Albadran
Titanium dioxide nanoparticles (TiO2 NPs) is an important industrial material that is widely used as an additive in cosmetics, pharmaceuticals, and food colorants. Although the small size of the TiO2 nanoparticles is useful in various applications, the biosafety of this material needs to be estimated .For this purpose this study was designed to evaluated the cytotoxic effects of TiO2nanoparticle on Rat Embryo Fibroblast cell line (REF-3) in vitro. Seven concentrations of TiO2 nanoparticles (0.5,1,5,10,25,50 and100 ) μg/ml were prepared and tested on cell line with three replicates for each concentration ,The optical density of cell growth read by Elisa reader 492 nm by used Tetra zolium Bromide (MTT).The result of REF-3cellgrowth assay had shown that the TiO2nanoparticles had cytotoxic inhibition ,This inhibition was time and dose dependent. the inhibitory effect of TiO2nanoparticles on the proliferation of REF-3started from concentration 1 μg/ml during 24 hour .The CC50% of TiO2nanoparticles on REF-3 cell line were more than 100μg/ml for all period of time
