Gene Editing Technology

Gene editing or Genome editing is a type of genetic engineering technology in which particular DNA is inserted, duplicated or replaced in the genome of living organism with the help of molecular scissors called engineered nucleases .Four different types of nucleases are used : meganucleases, zinc finger nucleases (ZFNs), transcription activator-like effector-based nucleases interspaced short palindromic repeats (CRISPR)-Cas system. genome editing through cell line engineering in human cancer cells and IPSCs, and also in mammalian model organisms such as knockout mice and rats is done in in vitro conditions. site-directed mutagenesis, recombination based methods DNA sequence of the target organism is modified by reverse genetic analysis technique.


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