Methicillin-resistance of Staphylococcus species in southern Benin: Resistance gene, virulence factor associated and staphylococcal chromosomal cassette distribution

Koudokpon Charles Hornel

doi:10.21767/2472-1093-C2-006

Methicillin-resistance of Staphylococcus species in southern Benin: Resistance gene, virulence factor associated and staphylococcal chromosomal cassette distribution

6^th Edition of International Conference on Antibiotics, Antimicrobials and Resistance

October 11-12, 2018 Edinburgh, Scotland

Koudokpon Charles Hornel

Doctoral School of Life and Land Sciences, France

Posters & Accepted Abstracts: J Infec Dis Treat

Abstract:

Methicillin resistance of staphylococcal strains remains a public health concern. The present study aims to detect the carriage of the mecA gene by staphylococcal strains and the associated virulence factors. Seventy-eight staphylococcal strains collected from three hospitals in southern Benin were identified in Maldi TOF MS. Antibiotic sensitivity was determined by antibiotic disk diffusion on Mueller Hinton-2 medium. The search for the mecA gene, the production of Panton-Valentine leucocidin (PVL), toxic shock syndrome toxin (TSST) and detection of staphylococcal chromosomal cassette were performed by real time–polymerase chain reaction. The results obtained show that Staphylococcus saprophyticus was the most isolated species in the urine (38.4%), followed by Staphylococcus aureus (21.8%), Staphylococcus sciuri (21.8%), Staphylococcus conhii (6.4%), Staphylococcus heamolyticus (1.3%), Staphylococcus hominis (1.3%) and Staphylococcus xylosus (1.3%). In the cervico-vaginal secretion specimens, Staphylococcus aureus was the most isolated with a proportion of 5.1%. From the study of antibiotic sensitivity, there is a strong resistance of strains to beta-lactams, but no resistance to glycopeptides. 24.4% of the staphylococcal strains harbored the mecA gene. Between them, 52.4% of the Staphylococcus aureus strains were carriers of the mecA gene and 41.2% of the Staphylococcus sciuri strains also carried them. 33.3% of strains of Staphylococcus aureus produced PVL and 14.3% produced TSST. Two types of cassettes were identified in Staphylococcus aureus ccrB IV (n = 3) and ccrC (n = 4). Only one type of cassette was found in the strains of Staphylococcus sciuri bearing the mecA gene, that is ccrB II (n = 1). It is therefore important to initiate the search for these genes routinely for the proper care of patients.