Fengjiao Fan, Maolin Tu, Meng Liu, Pujie Shi, Yun Wang, Di Wu and Ming Du
Harbin Institute of Technology, China Dalian Polytechnic University,China
Posters & Accepted Abstracts: J Food Nutr Popul Health
Lactoferrin is reported to be a potential food protein with osteogenic activity. However, the activity of lactoferrin peptides is questionable. In the present study, we isolated and characterized peptides from lactoferrin with stimulatory effect on osteoblast proliferation. Peptides from the lactoferrin pepsin hydrolysate were puriï¬ed using cation-exchange and gel-ï¬ltration chromatography. Effects of different hydrolysates and peptides on the proliferation of osteoblast MC3T3-E1 cells were compared by MTT assay. Results showed that fraction P5-a from Superdex Peptide 10/300 GL gel chromatography showed better activity. Tricine-sodium dodecyl sulfate polyacrylamide gel electrophoresis and high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry conï¬rmed that two peptides components of P5-a corresponded to fractions of 20−78 and 191−277 amino acids in Bos taurus lactoferrin molecule (GI: 221706349). These results will provide some theoretical and practical data for the preparation and application of osteogenic peptides in functional food industry.
Spanish 
Chinese 
Russian 
German 
French 
Japanese 
Portuguese 
Hindi 