Distributions of Invasive Weed Parthenium (Parthenium hysterophorus L.) in the University Campus Peshawar, Pakistan

A survey was performed to measure the distribution of invasive parthenium (P. hysterophorus) weed in the university campus, Peshawar, the capital city of Khyber Pakhtunkhwa province in 2016. The University Campus included: The University of Peshawar, Islamia College University, The University of Agriculture Peshawar, Pakistan Forest Institute and the New Developmental Research Farm, The University of Agriculture Peshawar were the main studied areas. In the selected areas P. hysterophorus distribution was measured in the academic, residential and research farm areas. Moreover, the survey also investigated the impact of parthenium weed upon native weeds flora inside the University campus. A 1m2 quadrat was used to collect the data. During the survey, a total of 32 weeds species were recorded belonging to 18 different families and 32 genera, the life cycle of 23 weeds were annual while 9 weeds have perennial life cycle. The data were recorded on absolute density (%), relative density (%), absolute frequency (%), relative frequency (%) and importance values (%) of parthenium weed and others flora. The data showed that parthenium weed was most dominant species having 76.8 m-2 plants density in the NDF and (32.0 m-2) plants in Islamia University. However, the highest frequency (100%) of P. hysterophorus was observed for The University of Agriculture, while minimum (80%) occurred in Agronomy field. Moreover the relative frequency of P. hysterophorus was maximum (25%) seemed for Islamia College; whereas, its minimum (12.9%) at Agronomy field. Lastly, the Importance Value of Parthenium indicates the highest (45.98%) at Dairy farm followed by (40.33 %) at Horticulture field’s area. In conclusion, the Parthenium weed become more problematic and replaces all the native flora in the non-crop area specially Dicanthium annulatum a native grass specie on the on the ridges of the field being replaced by parthenium. Due to no proper management and high growth rate, nowadays it becomes as super weed in the KPK and especially in the Pakistan. So, the management of Parthenium weed requires the call attention from the Govt. policy maker and proper quarantine inspection to conserve the native flora and field crops to get maximum crop yield.


Introduction
Parthenium hysterophorus L. weed is an annual plant belongs to family Asteraceae. The plant can achieved height up to 2 meters when germinated in healthy soil and also have the ability to produce flowers after 4-6 weeks of germination [1]. A healthy and mature plant can produce up to 15000 seed plant-1 which could be transferred through moving water, animal's body, clothing vehicles, winds, machinery or birds [2]. In favorable environmental conditions, mostly the plant seeds germinate in two years [3], even though an important part of buried seeds have the ability to endure as dormant for numerous years [4]. For healthy germination of requiring alkaline to neutral clay soils [5], while could be grown on less effective on a various kind of soil natures. The water availability for this weed growth and germination comparatively more are inadequate by deprived rainfall [6].
Parthenium is a noxious and aggressive weed to South America and central tropical, and now days spreading to the subtropical and tropical content of the world [7]. In 1956, by chance the parthenium weed was introduced in India [8] and nowadays found everywhere of Indian sub-continent countries, like Pakistan. In Asia, the spreading of Parthenium weed very fast and reached to Vietnam, Taiwan and southern China [9], also reported in African countries like; South Africa, Kenya, Mozambique and Ethiopia and invasive for many Pacific Islands [10,11].
The invasive Parthenium creates problems for human health as well losses of agriculture yields. The Parthenium hysterophorus L. plant and its relative species have sesquiterpene lactones [12] which encourage the allergic dermatitis problems and other signs. Create problems as damage to human health, crop production, biodiversity and animal husbandry throughout the globe [13]. The invasive structure of Parthenium replacing the endangered local medicinal plant flora which utilized as medicine source [14]. Especially in India, Parthenium reduces the several crop yields up to 40% [15] while, up to 90% losses in forage production [9]. Another hand in Australia, found as a major threat to perennial grasslands in the central state of Queensland [1,4] whereas losses in beef production up to AU$22.0 million per year [16]. Parthenium, now introduces himself as cropland weed in Pakistan [13]. For domestic animals it is inedible, but sometimes sheep and cattle will eat in feed limitation or rare.
Globally, the high costs of herbicides prohibit their use for Parthenium weed control. Management will be a success when growing fine pasture grass which has the capability to compete with Parthenium. In the more severe situation of the crop, Parthenium weed can be controlled by application of 2, 4-D or Atrazine [17]. Bio-agents is well substituted to the chemical for perennial grasslands, which is the natural management of Parthenium could be in progress especially in Australia etc.
This survey looks at the current occurrence of Parthenium weeds inside the primitivity of Peshawar University Campus and in the New Developmental Research Farm, The University of Agriculture Peshawar, Pakistan.
In spreading, soil as seed banks is reported for seed dormancy, seed longevity and the capability of weed to grow in the favorable environmental conditions. From the previous investigation, the dormancy of seeds in soil was resolute to be high (44639 seeds m -2 ) Queensland [18] and soil seed bank persistency reached to 65-87%. The seedling of Parthenium invasive weed rapid growing observed from soil samples as compared to other plants species. Keeping in view the importance of this invasive weed in term of suppressing native flora a comprehensive survey was conducted in the University campus with the following objectives.
• To identify the weed flora of University campus, Peshawar • To find out the most infested university among the three studied universities regarding weeds population • To compute the infestation of invasive Parthenium weed around the University Campus, Peshawar. In selected locations, two areas along the field were randomly selected and then surveyed. The placement of five (1x1 m 2 ) quadrats was randomly done in a horizontal pattern, in each location. In placed quadrats, the spaces among each quadrate were depending upon the availability of the area along the field and any obstructions that may have been present. The identification of collected weed was done in the Department of Weed Science, The University 0f Agriculture Peshawar, Pakistan (Figure 1).

Results and Discussion
Interestingly in the total of 32 Weeds species were identified and collected, belonging to 18 families and 32 genera. 23 weeds have annual life cycle and 9 weeds have perennial. Distribution of annual weed nature shows that few of the annual weeds were found the road sides while majority in the fields as the field have average rotation of two crops mostly which flourish the growth of annual weeds while the perennial weeds mostly grown on the ridges, the only dominant species in all the field area non field area mostly ridges was parthenium which mostly displayed the native species specially Dicanthium annulatum a native weeds specie become extinct now a days on the ridges and road sides. Moreover the study also shows that parthenium is susceptible to field disturbance and other field relative operations i.e tillage, ploughing. Most of the crop with great density have no parthenium weed recorded while crop growing on the ridges with free spaces invaded by parthenium i.e maize fields. The overall distribution and frequency of all the weeds recorded were as shown in the table listed below (Table 1).

Absolute weed density
The data in illustrates that the variability in weed dynamics throughout the 10 studied sites. In absolute weed density resultant data, the utmost distribution of Parthenium weed (76.8 m 2 ) was confirmed at Dairy farm, the University of Agriculture. Whilst, the distribution of Parthenium at Agronomy field, New Academic Block, Horticulture fields, and Plant Breeding and Genetic engineering fields were infested with the amount of (59.2, 58.4, 50.4 and 44.8 m 2 ) respectively. However, except of Parthenium weed, the infestation of Cynodon dactylon L., at Islamia Collegiate School with 100 plants m 2 were noted. In studied sites, the calculated mean of all weed species bring to light, the Cynodon dactylon L. 50.56 m 2 was most predominant species. Apart from that, the Parthenium (48.08 m 2 ) and Oxylis weed species infesting to the extent of 10.48 m 2 and all other areas which possessed the equal number. Moreover, the lowest absolute weed density in studied sites occurredat the Islamia Collegiate School, Islamia College University, Daubi Guard and New Academic Block as the amount of (32, 36.8, 38.4 and 41.6 m 2 ). The Parthenium weed acceleration was observe in noncropping area as well up to some extend in agriculture growing sites which inhibits the crop yields as well as replace other native flora. These findings are agreement of [20,21] who report that the distribution of invasive Parthenium, damage the agriculture crops and its production as well as agitate the ecosystem and cause human health (Table 2).

Relative weeds density (%)
As regards to relative weed density data (%) of all studied sites at the University Campus Peshawar, The aggrandize percent of relative weeds density (48.48%) for Dairy farm area, (35.20%) Horticulture field area, New Academic Blocks (33.33%), Agronomy field area (30.19%) and Forest Institute jungle area (25.24%) of flora comprised of Cynodon dactylon L. weed species respectively. However, relative density even surpassed the Cynodon dactylon L. at Islamia College University (36.41%) and Forest Institute colony Map with GPS Coordinates of the (studied area), where the distribution of parthenium and other weeds flora in the university campus, Peshawar.    The means data further showed the Cynodon dactylon L. was the more occurred species with 36.41% relative density and the Parthenium weed 25.00% and Oxylis Species 4.5%. In studied survey, observed the Parthenium distribution become more rapidly day by day due to its strong competitive nature. After Cynodon dactylon, the density of Parthenium is high as compare to others infested flora, it can be in non-cropping area as well as crop cultivated area. The dominancy of Parthenium weeds on local flora, due to the rapid infestation in wastelands or undisturb area. May be due to its invasive potential, high growth rate, more allelopathic properties, large quantity of seeds production and short growth cycle [22]. Same situation was surveyed, in rainy season the distribution and infestation of Parthenium and Achyranthes aspera L. weed are more dominant [23] (Table 3).

Absolute frequency (%)
The configuration of local flora in the studied locations at the Peshawar University Campus and the New Developmental Farm, The University of Agriculture Peshawar and Islamia College University. In absolute frequency of local flora, the higher frequency 100% of Parthenium weed was noted at the studied areas, the University of Peshawar and The University of Agriculture and Islamia College University. In spite of fact that, the lower absolute frequency Parthenium occurred in Agronomy field area 80% were absorbed and then Cynodon dactylon L., Taraxicum officinale L. and Convolvulus arvensis L. as the subsequent evenly infested species in the studied location. The absolute Frequency mean data of flora distribution showed the Parthenium and Johnson grass as more frequent weeds and then Euphorbia heliscopia, Poa annua, and Plantago lenceulata respectively. The Parthenium is an alien weed, commonly distributed in neotropical and pan-tropical distributed, Pakistan. The absolute frequency and spreading is very fast in degraded areas, wetlands, along the water bodies, railway tracks and roadsides as well as nowadays reported in crops cultivated field. Also, have negative effects on animal husbandry, crop production and native flora as well human health [24] (Table 4).

Relative frequency (%)
The distributed weeds relative frequency is a good statistic that indicates the infestation of weeds in the investigated location. The mean data of relative frequency show that the domination of Parthenium hysterophorus L. (20.28%) was calculated followed by Cynodon dactylon L. (15.55%) and Taraxicum officinale L. (5.92%) across all sites. The highest relative frequency of Parthenium occurred in Islamia College University area which is (25%) and then Cynodon dactylon L. also occurred in the same site. Tabulated data furthermore illustrates the Parthenium weed distribution was almost same in all the studied sites; though its relative frequency (12.9%) was lowest recorded at Agronomy field area (The University of Agriculture Peshawar) where there were a good weed management practices in Agronomy field. Moreover, Chickweed was noted with low (3.22%) relative frequency at Agronomy field. In survey, it was observe that the Parthenium weed relative frequency is low in cropping area due weed good management practice while, high in non-cropping area due undisturbed area. The highest relative frequency of Parthenium in the non-cropping area are too high as compare to agricultural cropping where always up to some extend weed management practices are in running [19]. This could have been predicted and is most likely due to this weed's highly competitive and allelopathic habit [25]. The displacement of native species may also be due to this weed's highly adaptive nature and because it is poorly grazed by cattle. Similar results to those being reported here for a decline in species richness follow in parthenium weed invasion have also been seen in India and Ethiopia [26,27] (Table 5).

Importance value (%)
The distributions of local flora at everywhere, the Importance   Table 3 Relative weeds density (%) for the selected sites of Peshawar University Campus.

Weed Species Common Names
The      Values of that play a key role. Among the studied locations, the mean of all the studied site the minimum (33.81%) IV was recorded for Parthenium followed by Cynodon dactylon (28.58%). Apart from that, the maximum Importance Value of 45.98% for Parthenium was founded at a Dairy farm and then 40.33% at horticulture field's area. Moreover, in the case of other than Parthenium weed, the Cynodon dactylon and Convolvulus arvensis were ranked as uppermost species in Islamia College and Forest Institute jungle as well as other all 10 studied areas. The collected data defecates the spreading of Parthenium weed in the studied locations, and its successful adjustable nature and it is quite inevitable to monitor the further infestation from infested to uninfected locations. Recently, majority of the rangelands and crops cultivated areas are infested with this invasive, noxious Parthenium weed, due to its fast growth and easily adjustability and dominancy on native flora [28]. The dormancy figure and fast infestation of Parthenium weed in forage, deceased up to 90%. Moreover, the presence of especially invasive weeds make land infertile and reduced the quality of grazing land, animal health, milk products as well meat [29,30]. P. hysterphorus had become a curse for the natural herbs of the Chhattisgarh plains in India.
In Islamabad, the Cannabis sativa is exotic invasive species, was once the most dominant weed species among the herbaceous flora [31]. It is now being replaced by P. hysterophorus, which is a