Aging is a natural phenomenon on that effects on different systems of our body. This process is not due to diseases and accidents. Activity of Daily Living (ADL) is important for elderly persons. The objective of this study was the effect of aerobic exercise on improvement of motor function in healthy elderly, after eight weeks of regular participation in walking. The 36 elderly female with age above 60 years old participated in this study. 18 elderly female in exercise group with mean age of 66.32 and 18 elderly female in control group with mean age of 67.22. The exercise group participated in aerobic exercise program for eight weeks. Functional 30-second chair stand test and 10-meter walk test were conducted to evaluate motor function. The Independent T-test and paired sample T-test was used to compare the differences between the pre and post-test in both groups. All statistical analyses were performed using SPSS statistical software version 19. Results showed significant difference in the assessed data of the exercise groups in the posttest level in comparison with the pretest level. Therefore, walking is appropriate for improve of motor function in elderly female.