Abstract

Study of in vitro antioxidant activity and HPTLC fingerprint of quercetin in Cassia auriculata L

The objective of the present study was to investigate the antioxidant activity of various parts of Cassia auriculata L., a species rich in antioxidants. The antioxidant competence of methanolic extracts of twigs, leaves and flower buds were screened using in vitro scavenging assays of 1,1-diphenyl-2-picryl hydrazyl (DPPH), superoxide, nitric oxide, hydroxyl, H2O2, lipid peroxides as well as reducing power and metal chelating abilities with reference to standards. The leaf extract showed significant higher lipid peroxide and DPPH radical scavenging activity exhibiting IC50 value of 8.902 μg mL-1 and 16.93 μg mL-1 as opposed to 3.623 μg mL-1 of ascorbic acid and 260.907 μg mL-1 of butylated hydroxyl toluene (BHT) respectively. Twigs exhibited maximum inhibitory activity on nitric oxide with IC50 26.60 μg mL-1 compared to 122.93 μg mL-1 of gallic acid. Preliminary phytochemical analysis revealed the presence of alkaloids, saponins, flavonoids, phenolics, glycosides, tannins and steroids. Flower buds exhibited higher phenolic content of 237.83 mg gallic acid equivalents g-1 of extract and twigs showed maximum flavonoid content of 136.23 mg quercetin equivalents g-1 of extract. HPTLC analysis of plant parts indicated the presence of the most abundant dietary flavonol, Quercetin.


Author(s): Jyothi S. Gowda* and Somashekaraiah B. Veerabhadrappa.

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