For patient management and therapeutic initiation, the rapid method of diagnosing leptospirosis is important. The microscopic agglutination test (MAT) is the gold standard serological test used in the reference laboratories with high sensitive and specific. In this study, the survey was done in order to understand the leptospiral epidemic in Coimbatore. One hundred and ten serum samples were included in this study from patients with suspected leptospirosis and were subjected to IgM Enzyme Linked Immuno Sorbent Assay (ELISA), Microscopic Agglutination Test (MAT). A battery of 10 predominant infecting serovars was included as MAT antigen. Among 240 cases, 213 serum samples showed positive results to MAT and predominant serogroup was Grippotyphosa (69%), followed by Autumnalis (17%), Australis (10%) and Patoc (4%). IgM ELISA showed a sensitivity of 97.3% and specificity of 100% compared to MAT. Among the serovar included in this serological study, Grippotyphosa was observed predominant and provide 100% specificity in diagnostics.