Suman Tiwari, Rakhi Tiwari, Richa Srivastava, Pratibha Kumari and Sujeet Kr. Singh
Random Amplified Polymorphic DNA (RAPD) polymorphism was employed to assess the genetic variations in the germplasm of Eucalyptus tereticornis. 20 trees under cultivation were analyzed with 10 RAPD primers of which all shows polymorphisms. Total genetic diversity (H) varied between 0.18 and 0.42 with an average of 0.35. Shannon's Information index (I) varied between 0.50 and 0.61, with an average of 0.52and overall genetic variation is 35%. Marker attributes like Polymorphism Information Content (PIC), Effective Multiplex Ratio (EMR), Marker Index (MI) and Resolving power (Rp)values were calculated to assess the discriminatory power of 10RAPD primers used. The PIC values ranged from 0.24 to 0.43 with an average of 0.34 per primer and the EMR ranged from 25 to 121 with a mean of 60.40 across all genotypes and the MI value ranged from 6.01 to 42.30 with an average of 19.55 per primer. The Rp values ranged from 20 to 140 with an average of 74 per primer. The highest value of similarity coefficient of 0.84 was found between the e-9 and e-1 while the lowest value of similarity coefficient of 0.20 was observed between the genotypes e-14 and e-11. The UPGMA-phenogram categorized the 20 trees into two major clusters based on genetic similarity and dissimilarity.
