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Molecular characterization of thermostable DNA polymerase of Bacillus stearothermophilus spp isolated from soil in Bangalore, India

Thermostable DNA polymerases are extensively used in DNA amplification reactions such as the Polymerase Chain Reaction (PCR), require the activity of the enzymes at high temperatures. The aim of the present study was to evaluate the probable biotechnological potential of Indian thermostable DNA polymerases. As a result, in the present study, DNA polymerase gene isolated from Bacillus stearothermophilus and amplified using Primer 3 plus software was ligated with T vector (pTZ57R/T) and transformed into DH5α cells. The plasmid DNA obtained, was then confirmed by restriction digestion and sequence analysis. The sequence was found to be 99% similar to a published sequence (GenBank sequence in NCBI). The study also suggests that the optimization of the enzyme activity might increase its use in PCR methods further.

Author(s): Atheer Hasan Atiyah Al Abedi and Selvam Arjunan

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