Commercially viable protocol for in vitro propagation of Chlorophytum borivilliens was achieved through direct organogenesis from shoot apex explants. Cultures were initiated on MS medium supplemented with 2 mg l-1 benzyl adenine (BA). From one shoot apex, 250 shoots proliferated when it was transferred to MS medium supplemented with 3 mg l-1 benzyl adenine in six months time. Ninety eight percent of the regenerated shoots rooted within a month on MS medium supplemented with 0.05 mg l-1 naphthalene acetic acid. Rooted plants showed 100 % survival on transfer to in vivo conditions. From one plant in 7 months, 2200 plants were micropropagated and ready for field transfer.