Romero-Arenas O, Rivera-Tapia JA, Damián Huato MA, Villareal Real OA,Márquez Specia M and López Olguín FJ
One of the substrates used to reproduce a Trichoderma spp., is whole grain rice, which has relatively high cost. In order to find a cheap and easy substrate acquisition in Tetela de Ocampo region of Mexico Puebla, where this fungus has a good development and a high production of viable spores was established this research aimed to assess five agricultural substrates mass reproduction and viability of spores of four strains of Thrichoderma native and associate it with the nutritional composition of the substrates used. Were evaluated grain of birdseed, rice, lentils, corn and wheat, we used a completely randomized design, with five replicates per treatment. We quantified the number and percentage of spore viability and correlated with the proximate analysis. The ground corn substrate was the best substrate; both in the production and germination of spores of strain TS1P1 with 117.5 PEx104/ml and 99.0% viability, respectively the substrate lentil grain present the second best production 94.38 PEx104/ml and 98% viability with the same strain. Based on the proximate analysis, the development of the mycelium, sporulation and spore viability, the native strains of Trichoderma spp., have great fit those agricultural substrates with high moisture, low in minerals, protein and fat
