European Journal of Experimental Biology Open Access

  • ISSN: 2248-9215
  • Journal h-index: 45
  • Journal CiteScore: 34.35
  • Average acceptance to publication time (5-7 days)
  • Average article processing time (30-45 days) Less than 5 volumes 30 days
    8 - 9 volumes 40 days
    10 and more volumes 45 days
Reach us +32 25889658

Abstract

Isolation, Purification and Estimation of Zeatin from Corynebacterium aurimucosum

Pooja P. Patel, Purvi M. Rakhashiya, Kiran S. Chudasama and Vrinda S. Thaker

Plant pathogenic organisms are known to affect plants by secreting enzymes, toxic compounds and phytohormones (IAA, KIN, GA, Zeatin and ABA). In the present study, bacteria were isolated from infected Prunus salicina. It was identified by biochemical tests and rDNA sequencing. It was studied for production of zeatin, a phytohormone known to influence plant growth and development. Conditions for zeatin production were optimized and after 10 days of incubation zeatin was extracted from the broth. The extract was subjected to isolation of zeatin using TLC. The further purification of zeatin was performed using HPLC and tested by immunoassay.