The present work discusses new and simple preparative TLC method for isolation of quercetin-3-O-β-D-glucoside from aqueous extract of leaves of Azadirachta indica. The purity of isolated quercetin-3-O-β- D-glucoside was 98.23 %. Structure of isolated quercetin-3-O-β-Dglucoside was established by various spectral techniques such as IR, NMR and mass spectroscopy. Isolated quercetin-3-O-β-D-glucoside can be used as marker compound for marker-based standardization of extracts and formulations containing Azadirachta indica leaves.
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