Advances in Applied Science Research Open Access

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Abstract

Isolation and identification of soil mycoflora in different crop fields at Salur Mandal

G. Gaddeyya, P. Shiny Niharika, P. Bharathi and P. K. Ratna Kumar

A total of 15 species belonging to 6 genera of fungi were isolated from agricultural fields at Salur Mandal during March 2011 to November 2011 in three intervals. The mycoflora were isolated by using soil dilution technique and soil plate technique on Potato Dextrose Agar and Czapek,s Dox Agar medium supplemented by suitable antibiotics such as penicillin and streptomycin. Identification and characterization of the mycoflora were made with the help of authentic manuals of fungi. The most common among them viz; Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Aspergillus nidulans, Aspergillus terreus, Penicillium chrysogenum, Penicillium frequentans, Penicillium funiculosum, Trichoderma viride, Trichoderma harzianum, Fusarium oxysporum, Fusarium solani, Curvularia clavata, Curvularia lunata, and Rhizopus stolanifer were isolated and characterized. The seasonal variation and percentage frequency of the mycoflora were statistically analyzed