The purpose of this study was to determine the occurrence of candida species in women referred to gynecology clinics (Tehran, Iran) with symptoms of vaginal candidiasis.150 vaginal swabs were collected from women with vaginal candidiasis. Then samples were cultured on Sabouraud Dextrose Agar for morphologic analysis. Genomic DNA of all clinical samples were extracted by glass bead and lysis buffer. PCR was performed to amplify ITS1- 5.8SITS2 in candida genome using universal primers. For final identification of candida species the PCR-RFLP was done. The result of this study showed that there were candida species in vagina of 110(73%) of the patients and four species of candida were identified. By using molecular method including: Candida albicans(87.2%), Candida labrata(10%), Candida tropicalis(1.8%) and Candida parapsilosis. (1%). According to our results however C. albicans was the most frequently isolated species (82.2%), nowadays the occurrence of the other non-albicans isolates such as Candida glabrata is increasing in vaginal candidiasis. The identification of candida species in vaginal candidiasis due to developing antifungal resistance is very significant for appropriate treatment and to prevent the spread of recurrent candidiasis in women.
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