Jagdish Singh and Rinkey Sharma
Saccharomyces crevisiae was treated with physical (ultraviolet and microwave irradiation), and chemical mutagen (5- bromouracil, allylthiourea, dithiotritol, acriflavin and acridine) to develop mutant with high ethanol producing efficiency. Two mutant, S. crevisiae SUVaM1, SCD10M3 produced high ethanol yield exceeding that of the wild type. Both wild and mutants show ethanol tolerance up to 10%. Maximum substrate tolerance for wild and SUVaM1 was10% but mutant SCD10M3 has 20% glucose. The best pH and temperature was 6 and 300C respectively for ethanol production for wild & mutants. This study also investigated the growth kinetics and ethanol productivity with Monod model. The resulting ethanol yield was 2.9-fold more than that of the wild type strain.
