In this study, the bioactive compounds of Enicostemma littorale have been evaluated using GCMS. The chemical compositions of the whole plant methanol extract of Enicostemma littorale were investigated using Perkin-Elmer Gas chromatography-mass spectrometry. The mass spectra of the compounds, found in the extract was matched with characterization and measurement of the central Electrochemical Research institute. GC-MS analysis of E.littorale whole plant methanol extract revealed the existence of the ether compound-Laminaribiitol (79.93%), 12-hydroxy-9-octadecenoic acid (9.546%). Myricetin (4.7519%), 3.3-Methylenebis (4- hydroxycoumarin) (2.811%), catechin (2.002%). The results of this study offer a base of using E.littorale as herbal alternative for the synthesis of antifungal agents.