All parts of the plant Vitex negundo L are known to have wide array of therapeutic activities and their essential oil is no exception. The present study was carried out to assess the chemical composition of the essential oil of the plant. The essential oil was obtained by hydro-distillation of fresh leaves of the plant in Clevenger type apparatus for approximately 8 hrs (Yield being 0.1%v/w). The extracted oil was analyzed using GC and GC/MS analysis. A total of 48 compounds were detected, of which 19 compounds represented 88.65% of the oil. The Epiglobulol was found as a major component having 30.31% concentration. Some other compounds detected in considerable range were delta-iraleine (10.34%), terpinen-4-ol (9.42%), gamma-elemene (5.72%), manool (5.16%), beta-iso-methyl ion (4.46%), beyerene (3.79%), and phytol (2.33%).
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