The effect of using creatine on aerobic activities has less been noticed. The data suggests that creatine increases the in use substrate during aerobic activities and probably leads to an improvement in aerobic performance. The main purpose of this research is analyzing the effect of creatine supplementation combined with aerobic training on anaerobic threshold in young men. The following study is a quasi-experimental study. The population of this study is formed by young healthy men of Shahrood city who do recreational activities. The primarily samples were volunteer selected. A group of 32 young healthy men (between the ages of 18 to 30) agreed to participate in this research. The study was done in 4 weeks and the data needed for a four-week-course was collected before and after the test. The statistical analysis was performed by “SPSS ver.16”. To analyze the data, ANOVA variance test was used. The results showed that using creatine over the course of four weeks, along with aerobic exercise, has effected on vo2 max (p=0.001). Therefore, it can be concluded that creatin supplementation probably have significant effects on VO2 max after 28 days aerobic exercise and consumption of creatin supplementation.