The human immune system is affected by exercise and physical activity. Plasma concentration of IL-6 increases more than other cytokines through exercise. Each of the training methods together with nutritional supplementation has its own specific effect on serum IL-6 expression. The purpose of this study is to confirm the impact of training bouts and CHO supplementation on serum IL-6. 19 male subjects with a mean age of 37.2 ± 24.89, BMI 6.97 ± 72. 25 kg, height 176.1 ± 4.60 cm, fat percentage, ± 5.80 1.18 kg and Vo2max 56.40 ± 1.74 kg liters per minute were present in two PLA or CHO groups. The test was completed in two days. Each of the subjects ran 60 minutes each day on the treadmill, with 80% MHR. On each day blood samples were collected from subjects 10 minutes before the exercise and then immediately after the exercise. The subjects drank either PLA or CHO drinks during exercise. The analysis of the samples was done by ELISA method. Data analysis was done in the form of descriptive and inferential statistical methods. To make a decision as to acceptance or rejection of hypotheses, the significance level of 0.05 was considered. IL-6 levels increased significantly in both groups under the influence of exercise and this increase was higher in PLA than in CHO; moreover their difference was significant. 24 hours after exercise, IL-6 serum concentrations in the two groups differed but the difference was not significant. Also under the influence of the second bout of exercise IL-6 serum in PLA group had a significant increase compared with the first day before the exercise (p <0.05). According to the findings of the research CHO supplementation during 60 minutes of running with less than 80% MHR causes less increase in the incidence of serum IL-6 in a delayed manner and immediately after exercise. Although by consumption of CHO, IL-6 levels increased after the first bout like Pla group, its amount was a smaller; also in repeated bout of exercise, serum IL-6 increase was not significant in CHO group, while this increasing trend in Pla group also continued after the second bout of exercise.