Marjaneh Sedaghati, Hamid Ezzatpanah, Massod Mashhadi Akbar Boojar, Maryam Tajabadi Ebrahimi and Mehdi Aminafshar
The aim of this work was to evaluate the presence of antibacterial properties in a plasmin digest of bovine �?�??�?�?�-casein. Although native bovine κ-caseinis resistant to plasmin, extensive hydrolysis of this milk protein was observed applying a long incubation period. The antibacterial potential of κ-casein, plasmin, PD�?�??�?�?� was evaluated against pathogenic (Escherichia coli and Staphylococcus aureus) and probiotic (Lactobacillus casei and Lactobacillus acidophilus) bacteria in vitro. Although �?�??�?�?�-casein and plasmin had no antibacterial activity, PD�?�??�?�?� showed antibacterial property against the tested bacteria. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of PD�?�??�?�?� was determined for the target bacteria. The MIC and MBC of PD�?�??�?�?� against Escherichia coli was considerably higher than Staphylococcus aureus, Lactobacillus casei and Lactobacillus acidophilus bacteria. The growth curves alterations of target bacteria in the presence of PD�?�??�?�?� were monitored by turbidimetry in broth culture. The effect of PD�?�??�?�?� on lag time and maximum absorbance was more significant than slope of tested bacteria. In addition of growth curves, PD�?�??�?�?� has inhibitory effects on the plate count confirmation of tested bacteria. The maximum inhibitory effect of PD�?�??�?�?� was created in MIC concentration.
