The cuttlefish ink extracts was good antibacterial activities. Sepia pharaonis was assayed against selected human pathogens using agar well diffusion technique. Crude ink extract in hexane and column purified ink extract in diethyl ether shows maximum inhibitory effect against Pseudomonas aeruginosa, Staphylococcus epidermidis, Klebsiella pneumoniae. The minimum inhibitory concentration was found to be lower in Escherichia coli and lesser degree of inhibition was observed in the column purified fractions when compared to crude ink extract.
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