Abstract

Direct regeneration of HFRI-5 from nodes internodes and apical shoot explants

The genus Eucalyptus contains some of the most important commercial tree species in the production of raw material for the pulp and paper industry and for a future bioenergy and biorefinery industry. Attempts have been made to set up Eucalyptus regeneration system through in vitro technique. Tissue culture protocol of Eucalyptus HFRI-5 has been developed using juvenile meristematic tissues (leaves and nodal cuttings) as explants. Profuse number of multiple shoots were initiated on Murashige and Skoog’s (MS) medium supplemented with vitamins, hormones and coconut milk. Physiological and biochemical parameters of hormones on regeneration system of Eucalyptus were studied. Induction of callus from leaf discs and nodal cutting was found to be regenerated in the presence of coconut water after six to twelve weeks of culture. The texture and response of callus obtained from explants, against different combinations of hormones were studied. Different anti-oxidants were also tested on the media for overcoming the phenolic excudation. Light conditions were also found to be important for regeneration. This protocol will facilitate the regeneration of selected Eucalyptus hybrid HFRI-5.


Author(s): Natasha Mahajana*, Manoj Kumarb, Vikas Shrivastavaa, Vijayta Sharmaa  and Uma Bhardwaja*

Abstract | PDF

Share This Article