Abstract

Development and validation of an analytical method for simultaneous estimation of telmisartan and ramipril using reverse phase HPLC in bulk and dosage form

The present work is focused to develop and validate a simple, rapid, accurate, sensitive and specific, linear Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) method for the estimation of Telmisartan and Ramipril in tablet dosage form. The elution was carried out through INTERSIL C18 column (250*4.6mm and 5 μm) in isocratic mode, with mobile phase containing mixed phosphate buffer(pH3.0):ACN :Methanol (20:50:30 v/v/v%) at a flow rate of 1.0ml / min and monitored at 220 nm. Chromatogram showed peaks at a retention time of 3.033 for Telmisartan& 4.003 for Ramipril. The method is validated for system suitability, linearity, precision, accuracy specificity, robustness, LOD and LOQ. Recovery of Telmisartan and Ramipril is found to be in the range of 98.8%- 100.50% respectively. The LOD and LOQ for estimation of Telmisartan & Ramipril are found to be 1.63μg/ml and 0.399μg/ml, and 4.9μg/ml and 1.2μg/ml respectively. Proposed method can be successfully applied for the quantitative determination of Telmisartan and Ramipril in Bulk drug and Pharmaceutical dosage form.


Author(s): P. Sunil Kumar Chaitanya

Abstract | PDF

Share This Article
Awards Nomination 17+ Million Readerbase
Google Scholar citation report
Citations : 4713

Der Pharmacia Sinica received 4713 citations as per Google Scholar report

Abstracted/Indexed in
  • Google Scholar
  • Genamics JournalSeek
  • China National Knowledge Infrastructure (CNKI)
  • Directory of Research Journal Indexing (DRJI)
  • Proquest Summons
  • MIAR
  • International Committee of Medical Journal Editors (ICMJE)
  • Serials Union Catalogue (SUNCAT)
  • Geneva Foundation for Medical Education and Research
  • Secret Search Engine Labs
  • CAS (Chemical Abstracting Services)
  • University of Barcelona

View More »

Flyer image