Research Journal of Oncology Open Access

  • Journal h-index: 4
  • Journal CiteScore: 0.30
  • Journal Impact Factor: 0.24
  • Average acceptance to publication time (5-7 days)
  • Average article processing time (30-45 days) Less than 5 volumes 30 days
    8 - 9 volumes 40 days
    10 and more volumes 45 days

Abstract

Caspase-3 levels (CASP-3) in Doxorubicin Induced-Cardiotoxicity: Role of Metformin Pretreatment

Hayder M Al-Kuraishy and Reem Ghanim Hussein

Background: The greatest danger of doxorubicin-induced toxicity is cardiotoxicity hence; administration of doxorubicin should be dose-limited. There are several biomarkers used to detect the cardiotoxicity which are cardiac troponin and caspase-3.

Aim: The aim of present study was assess caspase-3 serum levels in acute doxorubicin induced-cardiotoxicity in rats regarding the protective role of metformin.

Materials and methods: 18 Male Sprague Dawley rat were used in this experimental study. The rats were divided into three groups these were: Group �?�?�?�: Six rats as control group received normal saline 5 ml/kg/day intra-peritoneal for ten days. Group �?�?�?��?�?�?�: Six rats treated with normal saline 5 ml/kg/day intra-peritoneal for ten days and doxorubicin 20 mg/kg was given as a single dose intra-peritoneal at 8th day. Group �?�?�?��?�?�?��?�?�?�: Six rats treated with metformin 50 mg/kg/day orally via gavage for 10 days and doxorubicin 20 mg/kg was given as a single dose intra-peritoneal at 8th day. cTn-I serum level and caspase-3 serum level were assessed by specific ELISA kit method.

Results: Doxorubicin increase caspase-3 and cardiac Troponin I levels significantly p<0.01 compared to the control. Pre-treatment with metformin led to significant reduction in cTnI and caspase-3 serum levels compare to doxorubicin group p<0.05.

Conclusion: In doxorubicin induced-cardiotoxicity, caspase-3 is increased and regarded as a surrogate biomarker and pre-treatment with metformin produced significant cardio-protection in rats through reduction of caspase-3 serum levels.