Thura A. Kadhim
DNA is the central molecule in cells. The correct function of cells depends on the structure of DNA, and any mutation led to drives a wave of cellular multiplication associated with gradual increases in cancer occurring. The effect of paracetamol on DNA was studied using proliferative lymphocyte in vitro. DNA fragmentation was measured by quantitative method using (DPA). This study showed, that the Paracetamol drug at all concentrations (500μg/ml; 250μg/ml; and 125μg/ml) showed potent induction of DNA fragmentation in DNA after different exposure periods (24hrs; 48hrs; and 72hrs) respectively. Current results showed, that the paracetamol at 500μg/ml had highly apoptotic activity against normal lymphocytes,the percentage of fragmented DNA was 47.13 %, 69.32%, and 72.81% at 24hrs, 48hrs, and 72hrs respectively. The results showed significant effect (p< 0.05) of paracetamol on treated cells at 250μg/ml after 24hrs of exposure time, the percentage of DNA fragmentation was 51.94%, 60.00% and 57.89%, after 24hrs, 48hrs, and 72hrs of exposure time, respectively. However, the ability of paracetamol at low concentrations (125 μg/ml) were achieved a significant fragmentation (p ≤ 0.05) on lymphocytes DNA; percentages of DNA fragmentation was 44.76%, and 61.66%, and 70.17% respectively after 24hrs, 48hrs, and 72hrs of incubation period in. Indeed, the inhibition rate was increased with increasing time of incubation. On the other hand, present results showed low percentage of DNA fragmentation (21.14%, 21.78%, and 23.42%) in untreated cells after different exposure periods (24, 48, and 72hrs) respectively.
