European Journal of Experimental Biology Open Access

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Abstract

Antibiotic resistance pattern of bacteria spp isolated from hospital waste water in Ede South Western, Nigeria

Bolaji A.S, Akande I.O, Iromini F.A, Adewoye S.O and Opasola O.A

This study was carried out to investigate the presence of antibiotic resistance bacteria in hospital waste water collected from selected private hospitals in Ede, Southwestern, Nigeria. The bacteria isolates were identified to be Enterobacter aerogenes, Pseudomonas putida. Pseudomonas fluorescens, Klebsiella edwardsii, Proteus mirabilis, Pseudomonas aeruginosa, Shigella spp and Flavobacterium meningosepticum. Antibiotic susceptibility of the bacteria isolates was assayed according to the Kirby – Bauer disc diffusion method. The result of this study indicated that strains of bacteria isolated in this study have become resistant to all the tested antibiotics, and this showed that they have become multi-resistant to these therapeutic agents. This is obvious from the results of percentage of the isolates that are resistant to all the tested antibiotics, from the results it was observed that Enterobacter aerogenes, Pseudomonas putida. Pseudomonas fluorescens, Klebsiella edwardsii, Proteus mirabilis, Pseudomonas aeruginosa, Shigella spp and Flavobacterium meningosepticum were all 100% resistant to Septrin (30μg), Chloramphenicol (30μ), Amoxicillin (30μg)and Streptomycin (30μg) while they were also 90% resistant to Pefloxacin (10μg), Tarivid (30μg) ,80% resistant to Ciprofloxacin (10μg) 70% resistant to Gentamycin (10μg).This result showed that these organisms have been well exposed to the tested antimicrobials and they have developed mechanisms to avoid them. This study showed that antibiotic resistant bacteria are also present in Hospitals where people go to for the treatment of infections.