Advances in Applied Science Research Open Access

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Abstract

A correlation between fluorescence of chlorophyll solution in some medicinal plant leaves and absorption in liquid phase

Mitali Konwar

Red chlorophyll fluorescence of a number of medicinal plants Azardicta indica, Clerodendron colebrookonium, Leucas linifolia, Mesua ferua, Leucas cephalotes/lini folia have been photographed with the help of a two prism glass spectrograph and a 500W hellogen lamp as continuum. The corresponding absorption spectra of this sample have also been photographed. The salient points of the observation are that the fluorescence intensity depends on the concentration of the sample but at the same time it also depends on the absorption of the sample. When the red fluorescence intensity is maxima the absorption band also exhibits maximum extinction. The fluorescence intensity has been estimated with the help of a photodiode connected to a optical fiber. The result so obtained in the work can be used to estimate the fluorescence content of the solution of the medicinal plants. We have also used Ar+ laser to excite fluorescence spectra and worked out a correlation between absorption and fluorescence.